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| Registros recuperados: 59 | |
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| Dupont, Samuel; Lokmer, A.; Corre, E.; Auguet, Jean-christophe; Petton, Bruno; Toulza, E.; Montagnani, Caroline; Tanguy, G.; Pecqueur, D.; Salmeron, C.; Guillou, L.; Desnues, C.; La Scola, B.; Bou Khalil, J.; De Lorgeril, Julien; Mitta, Guillaume; Gueguen, Yannick; Escoubas, Jean Michel. |
| Background The impact of the microbiota on host fitness has so far mainly been demonstrated for the bacterial microbiome. We know much less about host-associated protist and viral communities, largely due to technical issues. However, all microorganisms within a microbiome potentially interact with each other as well as with the host and the environment, therefore likely affecting the host health. Results We set out to explore how environmental and host factors shape the composition and diversity of bacterial, protist and viral microbial communities in the Pacific oyster hemolymph, both in health and disease. To do so, five oyster families differing in susceptibility to the Pacific oyster mortality syndrome were reared in hatchery and transplanted into a... |
| Tipo: Text |
Palavras-chave: Oyster genetic background; Hemolymph microbiota dynamics; Early-life microbiota; Trans-kingdom interactions; Crassostrea gigas; Within-host ecosystem. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00627/73916/73254.pdf |
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| Buchet, Vincent; Suquet, Marc; Omnes, Marie-helene; Petton, Bruno; Normant, Yvon; Severe, Armelle; Quemener, Loic; Barone, Herve; Fauvel, Christian; Gaignon, Jean-louis. |
| The fishing of pollack which concerns essentially, France, United Kingdom and Norway, and at a lower level Ireland and Denmark, is situated between 10 and 15000 tons a year. In 1996, France was the first producer of Pollack. Since 1980, a decrease of production is observed in Europe. In France, landing reached 3000t. in 1998 (Suquet, 2001). However, the demand remaining strong, aquaculture could be a solution to satisfy the deficit between the French production and the domestic demand. |
| Tipo: Text |
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| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/00170/28144/26365.pdf |
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| Lemire, Astrid; Goudenege, David; Versigny, Tiphaine; Petton, Bruno; Calteau, Alexandra; Labreuche, Yannick; Le Roux, Frederique. |
| Disease in oysters has been steadily rising over the past decade, threatening the long-term survival of commercial and natural stocks. Our understanding and management of such diseases are of critical importance as aquaculture is an important aspect of dealing with the approaching worldwide food shortage. Although some bacteria of the Vibrio genus isolated from diseased oysters have been demonstrated to be pathogenic by experimental infection, direct causality has not been established. Little is known about the dynamics of how the bacterial population hosted by oysters changes during disease progression. Combining experimental ecology, a high-throughput infection assay and genome sequencing, we show that the onset of disease in oysters is associated with... |
| Tipo: Text |
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| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00244/35565/34107.pdf |
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| Foulon, Valentin; Artigaud, Sebastien; Buscaglia, Manon; Bernay, Benoit; Fabioux, Caroline; Petton, Bruno; Elies, Philippe; Boukerma, Kada; Hellio, Claire; Guerard, Fabienne; Boudry, Pierre. |
| Bioadhesion of marine organisms has been intensively studied over the last decade because of their ability to attach in various wet environmental conditions and the potential this offers for biotechnology applications. Many marine mollusc species are characterized by a two-phase life history: pelagic larvae settle prior to metamorphosis to a benthic stage. The oyster Crassostrea gigas has been extensively studied for its economic and ecological importance. However, the bioadhesive produced by ready to settle larvae of this species has been little studied. The pediveliger stage of oysters is characterized by the genesis of a specific organ essential for adhesion, the foot. Our scanning electron microscopy and histology analysis revealed that in C. gigas the... |
| Tipo: Text |
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| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00462/57379/59452.pdf |
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| Petton, Bruno. |
| Depuis 2008, des mortalités massives d’huîtres creuses âgées de moins d’un an sont relevées sur le littoral français dès que la température de l’eau de mer atteint le seuil de 16°C. Ces mortalités de naissains sont associées à la détection du virus OsHV-1 μVar. Des travaux de qualification zoo-sanitaire menés depuis 2010 ont montré qu’un lot de naissains issus du captage naturel sur deux était infecté par le virus bien avant l’apparition des mortalités dans le milieu naturel, mais qu’a contrario les naissains d’écloserie expertisés présentaient une fréquence plus faible de portage latent. Dans ce contexte de crise zoo-sanitaire chronique, l’objectif de notre étude consistait à obtenir une information du statut zoo-sanitaire OsHV-1 μVar des populations de... |
| Tipo: Text |
Palavras-chave: Huitre creuse; Naissain; Survie; OsHV-1 μ Var; Epreuve thermique.; Pacific oyster; Spat; Survival; OsHV-1 μVar; Thermal challenge. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00335/44586/44304.pdf |
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| Petton, Bruno; Riobe, Marion; Koechlin, Hugo; Queau, Isabelle; Mingant, Christian; Ratiskol, Dominique; Le Grand, Jacqueline; Petton, Sebastien; Thomas, Yoann; Alunno-bruscia, Marianne; Pouvreau, Stephane; Pernet, Fabrice; Maurer, Daniele; D'Amico, Florence; Lagarde, Franck; Mortreux, Serge; Roque D'Orbcastel, Emmanuelle; Mille, Dominique; Geay, A.; Bouquet, A.-l.; Cochet, Helene. |
| Depuis 2008, des mortalités massives d’huîtres creuses âgées de moins d’un an sont relevées sur le littoral français dès que la température de l’eau de mer atteint le seuil de 16°C. Ces mortalités de naissains sont associées à la détection du virus OsHV-1 μVar. Des travaux de qualification zoo-sanitaire menés depuis 2010 ont montré qu’un lot de naissains issus du captage naturel sur deux était infecté par le virus bien avant l’apparition des mortalités dans le milieu naturel, mais qu’a contrario les naissains d’écloserie expertisés présentaient un moindre risque de contamination. Dans le contexte de crise sanitaire chronique, l’objectif de notre étude était d’ obtenir une information qualitative relative au statut zoo-sanitaire OsHV -1 μ V ar des... |
| Tipo: Text |
Palavras-chave: Huitre creuse; OsHV - 1μVar; Epreuve thermique.; Maladie; Naissain; Pacific oyster; Spat; Disease; Thermal challenge; OsHV-1 μVar. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00276/38682/37180.pdf |
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| Petton, Bruno; Le Grand, Jacqueline; Riobe, Marion; Mingant, Christian; Ratiskol, Dominique; Petton, Sebastien; Alunno-bruscia, Marianne. |
| Depuis 2008, des mortalités massives d’huîtres creuses âgées de moins d’un an sont relevées sur le littoral français dès que la température de l’eau de mer atteint le seuil de 16°C. Ces mortalités de naissains sont associées à la détection du virus OsHV-1 μVar. Depuis 2010, des travaux de qualification zoo-sanitaire ont montré qu’environ un lot de naissains de captage sur deux était infecté par le virus avant l’apparition des mortalités dans le milieu naturel. En 2014, dans le cadre de la première action QUALIF, les résultats de la qualification zoo-sanitaire des naissains du captage de l’année 2013 à l’échelle nationale ont mis en évidence le caractère infectieux des maladies développées par ces naissains porteurs latents d’OsHV-1 μVar. L’objectif 2015 du... |
| Tipo: Text |
Palavras-chave: Huitre creuse; Naissain; Maladie; OsHV-1 μVar; Epreuve thermique.; Pacific oyster; Seed; Disease; OsHV-1 μVar; Thermal challenge.. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00335/44587/44305.pdf |
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| Fuhrmann, Marine; Petton, Bruno; Quillien, Virgile; Faury, Nicole; Morga, Benjamin; Pernet, Fabrice. |
| Mortality of young Pacific oysters Crassostrea gigas associated with the ostreid herpesvirus 1 (OsHV-1) is occurring worldwide. Here, we examined for the first time the effect of salinity on OsHV-1 transmission and disease-related mortality of C. gigas, as well as salinity-related effects on the pathogen itself. To obtain donors for OsHV-1 transmission, we transferred laboratory-raised oysters to an estuary during a disease outbreak and then back to the laboratory. Oysters that tested OsHV-1 positive were placed in seawater tanks (35‰, 21°C). Water from these tanks was used to infect naïve oysters in 2 experimental setups: (1) oysters acclimated or non-acclimated to a salinity of 10, 15, 25 and 35‰ and (2) oysters acclimated to a salinity of 25‰; the... |
| Tipo: Text |
Palavras-chave: Bivalve; Herpesviridae; Infection; Disease transmission; Risk analysis. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00350/46100/45781.pdf |
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| Holbach, Marine; Robert, Rene; Boudry, Pierre; Petton, Bruno; Archambault, Philippe; Tremblay, Rejean. |
| Pathogenic bacteria are known to be one of the main factors affecting Pecten maximus larval survival in hatcheries. As a result, juvenile production often relies on the use of antibiotics during larval culture. However, limitations of the usage of chemicals such as chloramphenicol in aquaculture have been reinforced due to their negative environmental impact and alternatives are accordingly needed. Thus, the importance of bacterial transfer from oocytes to larvae has been studied here as well as procedures to limit larval mortality in P. maximus. In order to reduce bacterial contamination during larval development, we focused on two periods, broodstock conditioning and post-fertilization. The animals were conditioned for 2 months with two erythromycin... |
| Tipo: Text |
Palavras-chave: Scallop; Broodstock conditioning; Larval survival; Vibriosis. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00240/35171/33675.pdf |
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| Piel, Damien; Bruto, Maxime; James, Adele; Labreuche, Yannick; Lambert, Christophe; Janicot, Adrian; Chenivesse, Sabine; Petton, Bruno; Wegner, K. Mathias; Stoudmann, Candice; Blokesch, Melanie; Le Roux, Frederique. |
| Pacific oyster mortality syndrome affects juveniles of Crassostrea gigas oysters and threatens the sustainability of commercial and natural stocks of this species. Vibrio crassostreae has been repeatedly isolated from diseased animals and the majority of the strains have been demonstrated to be virulent for oysters. In this study we showed that oyster farms exhibited a high prevalence of a virulence plasmid carried by V. crassostreae while oysters, at an adult stage, were reservoirs of this virulent population. The pathogenicity of V. crassostreae depends on a novel transcriptional regulator, which activates the bidirectional promoter of a Type 6 Secretion System (T6SS) genes cluster. Both the T6SS and a second chromosomal virulence factor, r5.7, are... |
| Tipo: Text |
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| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00510/62129/66343.pdf |
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| Rubio, Tristan; Oyanedel, Daniel; Labreuche, Yannick; Toulza, Eve; Luo, Xing; Bruto, Maxime; Chaparro, Cristian; Torres, Marta; De Lorgeril, Julien; Haffner, Philippe; Vidal-dupiol, Jeremie; Lagorce, Arnaud; Petton, Bruno; Mitta, Guillaume; Jacq, Annick; Le Roux, Frederique; Charriere, Guillaume; Destoumieux-garzon, Delphine. |
| Vibrio species cause infectious diseases in humans and animals, but they can also live as commensals within their host tissues. How Vibrio subverts the host defenses to mount a successful infection remains poorly understood, and this knowledge is critical for predicting and managing disease. Here, we have investigated the cellular and molecular mechanisms underpinning infection and colonization of 2 virulent Vibrio species in an ecologically relevant host model, oyster, to study interactions with marine Vibrio species. All Vibrio strains were recognized by the immune system, but only nonvirulent strains were controlled. We showed that virulent strains were cytotoxic to hemocytes, oyster immune cells. By analyzing host and bacterial transcriptional... |
| Tipo: Text |
Palavras-chave: T6SS; Toxin; Dual RNA-seq; Cytolysis; Pathogenesis. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00507/61837/65900.pdf |
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| Petton, Bruno; Pernet, Fabrice; Robert, Rene; Boudry, Pierre. |
| Since 2008, mass mortalities of 1-yr-old Crassostrea gigas associated with the ostreid herpesvirus OsHV-1 μVar have occurred along all the coasts of France when seawater temperature reaches 16 to 17°C. The present study aimed to characterize the effect of temperature on oyster survival in combination with OsHV-1 DNA quantification by standard real-time PCR and total vibrio population levels in oyster tissues. To examine the effect of seawater temperature on disease transmission and related mortality of oysters, cohabitation experiments were conducted between healthy naïve oysters and oysters previously exposed to field conditions in areas where mortalities were occurring. Oysters initially maintained in controlled conditions (free of mortality and negative... |
| Tipo: Text |
Palavras-chave: Juvenile oyster; Pathology; Temperature; Survival; Vibrios; Ostreid herpesvirus 1. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00148/25891/23912.pdf |
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| Castrec, Justine; Hegaret, Helene; Alunno-bruscia, Marianne; Picard, Mailys; Soudant, Philippe; Petton, Bruno; Boulais, Myrina; Suquet, Marc; Queau, Isabelle; Ratiskol, Dominique; Foulon, Valentin; Le Goic, Nelly; Fabioux, Caroline. |
| Harmful algal blooms are a threat to aquatic organisms and coastal ecosystems. Among harmful species, the widespread distributed genus Alexandrium is of global importance. This genus is well-known for the synthesis of paralytic shellfish toxins which are toxic for humans through the consumption of contaminated shellfish. While the effects of Alexandrium species upon the physiology of bivalves are now well documented, consequences on reproduction remain poorly studied. In France, Alexandrium minutum blooms have been recurrent for the last decades, generally appearing during the reproduction season of most bivalves including the oyster Crassostrea gigas. These blooms could not only affect gametogenesis but also spawning, larval development or juvenile... |
| Tipo: Text |
Palavras-chave: Harmful algal bloom (HAB); Paralytic shellfish toxin (PST); Crassostrea gigas; Gametes; Larvae. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00469/58099/60512.pdf |
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| Offret, Clement; Paulino, Sauvann; Gauthier, Olivier; Château, Kevin; Bidault, Adeline; Corporeau, Charlotte; Miner, Philippe; Petton, Bruno; Pernet, Fabrice; Fabioux, Caroline; Paillard, Christine; Blay, Gwenaelle Le. |
| Digestive microbiota provides a wide range of beneficial effects on host physiology and are therefore likely to play a key role in marine intertidal bivalve ability to acclimatize to the intertidal zone. This study investigated the effect of intertidal levels on the digestive bacterial microbiota of oysters Crassostrea gigas and clams Ruditapes philippinarum, two bivalves with different ecological niches. Based on the 16S rRNA region sequencing, digestive glands, seawater and sediments harbored specific bacterial communities, dominated by OTUs assigned to the Mycoplasmatales, Desulfobacterales and Rhodobacterales orders, respectively. Field implantation modified digestive bacterial microbiota of both bivalve species according to their intertidal position.... |
| Tipo: Text |
Palavras-chave: Microbiota; Oysters; Clams; Intertidal zone; Digestive gland; Metabarcoding. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00628/73961/73320.pdf |
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| Petton, Bruno; Destoumieux Garzon, Delphine; Pernet, Fabrice; Toulza, Eve; De Lorgeril, Julien; Degremont, Lionel; Mitta, Guillaume. |
| The Pacific oyster (Crassostreae gigas) has been introduced from Asia to numerous countries around the world during the 20th century. C. gigas is the main oyster species farmed worldwide and represents more than 98% of oyster production. The severity of disease outbreaks that affect C. gigas, which primarily impact juvenile oysters, has increased dramatically since 2008. The most prevalent disease, Pacific oyster mortality syndrome (POMS), has become panzootic and represents a threat to the oyster industry. Recently, major steps towards understanding POMS have been achieved through integrative molecular approaches. These studies demonstrated that infection by Ostreid herpesvirus type 1 µVar (OsHV-1 µvar) is the first critical step in the infectious process... |
| Tipo: Text |
Palavras-chave: Pacific oyster mortality syndrome; Polymicrobial disease; Multifactorial disease; Crassostrea gigas; OsHV-1; Opportunistic bacterial pathogens. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00679/79158/81669.pdf |
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| Bruto, Maxime; James, Adele; Petton, Bruno; Labreuche, Yannick; Chenivesse, Sabine; Alunno-bruscia, Marianne; Polz, Martin F.; Le Roux, Frederique. |
| Vibrios are frequently associated with oyster mortality; however whether they are the primary causative agent or secondary opportunistic colonizers is not well understood. Here we combine analysis of natural infection dynamics, population genomics and molecular genetics to ask (i) to what extent oysters are passively colonized by Vibrio population present in the surrounding water, (ii) how populations turn over during pathogenicity events and (iii) what genetic factors are responsible for pathogenicity. We identified several populations of Vibrio preferentially associated with oyster tissues. Among these, Vibrio crassostreae is particularly abundant in diseased animals while nearly absent in the surrounding water, and its pathogenicity is correlated with... |
| Tipo: Text |
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| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00360/47137/49044.pdf |
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| Registros recuperados: 59 | |
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